Isolation and properties of a pure bacterial strain capable of fluorobenzene degradation as sole carbon and energy source

摘要

A pure bacterial strain capable of aerobic biodegradation of fluorobenzene (FB) as the sole carbon and energy source was isolated by selective enrichment from sediments collected from a polluted site. 16S rRNA and fatty acid analyses support that strain F11belongs to a novel genus within the a-2 subgroup of the Proteobacteria, possibly within a new clade related to the orderRhizobiales. In batch cultures, growth of strain F11 on FB led to stoichiometricrelease of fluoride ion. Maximum experimental growth rate of 0.04 h-1was obtained at FB concentration of 0.4 mM. Growth kinetics were described by the Luongmodel. An inhibitory effect with increasing FB concentrations was observed, with no growth occurring at concentrations higher than 3.9 mM. Strain F11 was shown to be able to use a range of other organic compounds, including other fluorinated compoundssuch as 2-fluorobenzoate, 4-fluorobenzoate and 4-fluorophenol. To our knowledge, this is the first time biodegradation of FB, as the sole carbon and energy source, by a pure bacterium has been reported.